3X Flag Peptide Powder

3X Flag Peptide Powder Bulk Price GMP Manufacturer China | High-Affinity FLAG Peptide for Protein Purification Research with COA Verified Quality

Key Product Highlights:

• Triple FLAG epitope peptide (22 amino acids, tandem sequence)
• High-affinity binding to Anti-FLAG M2 antibody system
• Enables mild competitive elution under non-denaturing conditions
• Preserves native conformation and biological activity of recombinant proteins
• Minimizes non-specific binding and improves purification efficiency
• Applicable to protein purification, Co-IP, WB, and interaction analysis

3X Flag Peptide Powder is a synthetic 22-amino-acid peptide composed of three tandem FLAG epitope sequences. It is widely used as a standard molecular tool in protein purification and molecular biology research.

It specifically binds to Anti-FLAG M2 monoclonal antibodies and enables efficient competitive elution of FLAG-tagged recombinant proteins under gentle, non-denaturing conditions.

The product is manufactured under strict GMP conditions using solid-phase peptide synthesis (SPPS), followed by multi-step HPLC purification and ESI-MS structural confirmation to ensure high purity and sequence accuracy.

• High-affinity triple FLAG–antibody interaction
• Efficient and gentle competitive elution system
• Preserves native protein structure and biological activity
• Reduces background noise from non-specific binding
• Cost-effective China bulk supply for research procurement

• Purification of FLAG-tagged recombinant proteins
• Co-immunoprecipitation (Co-IP) experiments
• Protein–protein interaction analysis (pull-down assays)
• Western blot optimization and control studies
• Proteomics and target validation workflows
• Recombinant protein expression and purification systems

• High-precision solid-phase peptide synthesis (SPPS)
• Multi-step HPLC purification ensuring ≥98% purity
• Strict control of truncation peptides and impurities
• Excellent batch-to-batch reproducibility
• Full documentation: COA, HPLC chromatograms, MS reports
• Scalable production from milligram to gram quantities

The 3X FLAG Peptide Powder operates through a competitive antibody-binding mechanism.

During affinity purification, Anti-FLAG M2 antibodies immobilized on resin specifically capture FLAG-tagged fusion proteins. Upon addition of free 3X FLAG peptide, the peptide competitively binds to the antibody binding sites, displacing the target proteins under mild physiological conditions.

This process enables non-denaturing elution, preserving protein structure, enzymatic activity, and functional integrity, making it highly suitable for downstream biochemical and functional studies.

The peptide is stable in PBS and TBS buffers and does not interfere with protein conformation or assay performance.

• GMP-compliant cleanroom manufacturing environment
• Batch-wise HPLC purity and MS identity verification
• Endotoxin, microbial, moisture, and impurity testing
• Full traceability from raw materials to final product
• Strict QA/QC release procedures before shipment

Q: What is 3X Flag Peptide Powder used for?

A: It is used for purification of FLAG-tagged recombinant proteins, Co-IP experiments, and protein interaction studies.

Q: How does 3X Flag Peptide Powder work?

A: It works by competitively binding to Anti-FLAG M2 antibodies, gently eluting FLAG-tagged proteins under non-denaturing conditions.

Q: Can 1X Flag peptide elute 3X FLAG tagged proteins?

A: No, only 3X Flag peptide can effectively elute 3×FLAG fusion proteins from M2 resin.

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