Acriflavine Hydrochloride API CAS 8063-24-9 | High Purity Veterinary Pharmaceutical Ingredient Manufacturer & Bulk Supplier China

Key Selling Points

✓ Acridine Antiprotozoal + Antiseptic - DNA-Intercalating Dye

✓ Babesia-Specific Activity - Equine/Bovine/Ovine Piroplasmosis Treatment

✓ Broad Aquaculture Spectrum - Bacterial, Fungal & Protozoal Fish Diseases

✓ High Water Solubility (~330 g/L) - IV Injection & Bath Dip Ready

✓ Intrinsic Fluorescence - Dual Use as Antimicrobial & Biological Stain

✓ Trypaflavine Reference Molecule - Established Veterinary Antiprotozoal

What Is Acriflavine Hydrochloride Powder?

Acriflavine Hydrochloride Powder is an acridine-class compound - a mixture of 3,6-diamino-10-methylacridinium chloride hydrochloride and proflavine hydrochloride - historically known as Trypaflavine or Euflavine. It functions as both an antiprotozoal veterinary drug and a topical antiseptic, with additional applications as a fluorescent biological stain.

In large-animal veterinary medicine, acriflavine HCl is a well-established treatment for piroplasmosis (babesiosis) - it is active against Babesia caballi and B. equi in horses, B. bigemina and B. bovis in cattle, and Babesia species in sheep, administered by intravenous injection with clinical effect typically observed within 12–24 hours. It is important to note that acriflavine is not effective against Theileria or Anaplasma species. In aquaculture and ornamental fish keeping, it is widely used as a bath treatment for bacterial infections (Columnaris, Aeromonas), fungal infections (Saprolegnia, mouth fungus), protozoal ectoparasites (Oodinium / velvet disease), fin rot, gill disease and infected wounds or eggs. Its high water solubility (~330 g/L) makes it suitable for both injectable and aqueous formulations.

How Does Acriflavine Hydrochloride Powder Work?

Acriflavine Hydrochloride Powder exerts its antimicrobial and antiprotozoal effects through acridine-mediated DNA intercalation - the planar acridine ring system inserts between DNA base pairs, disrupting nucleic acid replication and transcription in susceptible microorganisms. This mechanism produces activity against Gram-positive bacteria (staphylococci, streptococci), protozoa (Babesia, trypanosomes) and certain fungi.

Because Acriflavine Hydrochloride Powder is a cationic dye with intrinsic fluorescence, it also serves dual utility as a nucleic acid stain and cellular analysis reagent in research laboratories. In veterinary therapeutic use, its DNA-binding property accounts for both its antiparasitic efficacy and its known cytotoxicity at high concentrations - it may reduce ATP production and increase lactate levels in exposed cells, which is why precise dosing and formulation control are essential. Its high aqueous solubility enables rapid IV distribution in large animals for babesiosis treatment, while bath or dip applications in fish deliver topical antimicrobial and antiprotozoal coverage without systemic exposure. As an acridine compound it is light-sensitive and strongly colored (orange-red), and it will stain aquarium decorations if not removed during treatment.

What Are the Applications of Acriflavine Hydrochloride Powder?

Equine Piroplasmosis: IV treatment of Babesia caballi and Theileria equi (formerly B. equi) infections in horses.
Bovine Babesiosis: Used against Babesia bigemina and B. bovis (Texas fever / redwater) in cattle via IV injection.
Ovine & Caprine Babesiosis: Treatment of Babesia infections in sheep and goats.
Aquaculture & Ornamental Fish: Bath treatment for Columnaris disease, Aeromonas infections, Saprolegnia fungus, Oodinium velvet disease, fin rot, gill rot, infected wounds and fungus-infected eggs.
Topical Antiseptic: Formulated into creams for fungal skin infections, wounds and burns in dogs, cattle and other animals (historical and adjunctive use).

What Is the Difference Between Acriflavine Hydrochloride and Neutral Acriflavine?

Feature	Acriflavine Hydrochloride	Neutral Acriflavine (Acriflavinium Chloride)
CAS	8063-24-9	8048-52-0
Form	Hydrochloride salt mixture	Neutral chloride salt
Water Solubility	Very high (~330 g/L)	Soluble
Skin Irritation	More irritating	Less irritating
Typical Use	IV injection / fish bath	Topical antiseptic / wound
pH	Acidic solution	Neutral

How Does Acriflavine Compare With Other Veterinary Antiprotozoals?

Feature	Acriflavine HCl	Diminazene Aceturate	Imidocarb Dipropionate	Quinapyramine
CAS	8063-24-9	536-71-0	27885-92-3	86-34-0
Class	Acridine dye	Diamidine	Carbanilide	Quinoline
Babesia	Yes	Yes	Yes	Yes
Trypanosoma	Yes (historical)	Yes	No	Yes
Theileria	No	No	No (some)	No
Anaplasma	No	No	Yes	No
Route	IV	IM	IM/SC	IM/SC
Fish Use	Yes	No	No	No

How Is Acriflavine Hydrochloride Powder Manufactured?

Acriflavine Hydrochloride Powder is manufactured through multi-step acridine synthesis involving condensation, amination, N-methylation and hydrochloride salt formation, followed by purification and drying.

Raw Material QC → Acridine Core Synthesis → Amination (3,6-diamino) → N-Methylation → Hydrochloride Salt Formation → Purification → Drying → Milling → Final QC → Packaging

HDM BIO controls key synthesis, amination, salt formation and purification parameters to maintain consistent purity, color intensity and batch quality.

Quality Specifications

Test Item	Standard	Typical Result
Appearance	Deep orange to brownish-red powder	Consistent
Assay (HPLC)	≥98%	99.0%
Identity (UV/IR)	Conforms to reference standard	Confirmed
Melting Point	179–181°C	180°C
Loss on Drying	≤5.0%	2.0%
Residue on Ignition	≤0.5%	Compliant
Heavy Metals	≤10 ppm	Compliant
Related Substances	Within specification	Pass
pH (1% solution)	2.0–4.0	3.0
Water Solubility	Clear solution	Compliant

What Quality Control Tests Are Performed for Acriflavine Hydrochloride Powder?

Each batch undergoes multi-step quality control before release to verify identity, purity, color and solubility.

Raw Material QC → In-Process Control → HPLC Assay → UV/IR Identity Verification → Melting Point Determination → Related Substances (HPLC) → Loss on Drying → Heavy Metals → Residue on Ignition → pH Test → Water Solubility → Final Batch Release

Key evaluations include identification via UV and infrared (IR) spectroscopy, HPLC active content assay, melting point determination, related substances analysis, loss on drying, heavy metal limits, residue on ignition, pH and water solubility testing. Every commercial batch is supported by a batch-specific COA and relevant analytical documentation for incoming QC and supplier qualification.

What Factors Affect Acriflavine Hydrochloride Powder Bulk Price?

Acriflavine Hydrochloride Powder bulk pricing is driven by several key factors:

Synthesis Costs: Multi-step acridine synthesis and N-methylation chemistry, including specialized raw material availability, directly influence manufacturing expenses.
Purity & Grade: Higher assay percentages and strict impurity control cost more to produce.
Regulatory Compliance & Certification: GMP-standard API batches with consistent COA and complete export documentation demand a higher price.
Order Volume: Large-scale industrial or wholesale contracts receive steeper per-kilogram discounts than small-batch specialty orders.
Logistics & Geography: Shipping distance, cold-chain / light-protection packaging requirements and customs compliance add to the landed cost.

Contact HDM BIO for a current quotation and volume-based pricing.

Why Choose HDM BIO as Your Acriflavine Hydrochloride Powder Supplier?

High-purity (≥98% / typical 99%) acridine antiprotozoal with proven Babesia activity
High water solubility (~330 g/L) suitable for IV injection and fish bath formulations
Broad aquaculture spectrum covering bacterial, fungal and protozoal fish diseases
GMP-aligned production, ISO & HACCP certified quality system
Full batch traceability and complete documentation (COA, MSDS, HPLC)
Flexible supply: gram samples to ton-level bulk orders
Global export to 70+ countries with full customs support

Bulk Packaging, Shipping & Storage

Packaging: Foil bags for samples and moisture-proof fiber drums for bulk orders.
Custom Packaging: OEM labeling available.
Shipping Documents: COA, MSDS/SDS, commercial invoice, packing list and customs documentation available according to order requirements.
Storage: Store tightly sealed in a cool, dry, light-protected environment. Shelf life: 24 months.
Handling: For veterinary raw material use only; not for direct clinical administration.

Frequently Asked Questions About Acriflavine Hydrochloride Powder

Q1: What is Acriflavine Hydrochloride Powder?

A: An acridine-class antiprotozoal and antiseptic (Trypaflavine/Euflavine), used in veterinary medicine for babesiosis treatment in horses, cattle and sheep, and as a bath treatment for bacterial, fungal and protozoal diseases in ornamental fish.

Q2: What is the CAS number?

A: 8063-24-9 (hydrochloride mixture); neutral acriflavinium chloride is 8048-52-0.

Q3: How does acriflavine work?

A: It intercalates into microbial DNA, disrupting nucleic acid replication and transcription. This produces activity against Gram-positive bacteria, protozoa (Babesia, trypanosomes) and fungi.

Q4: What parasites does acriflavine treat?

A: It is effective against Babesia species in horses, cattle, sheep and goats (piroplasmosis). It is NOT effective against Theileria or Anaplasma.

Q5: How is acriflavine administered?

A: In large animals by intravenous injection for babesiosis. In fish by bath or dip treatment in aquarium water. Topical creams are used for fungal skin infections.

Q6: What documents are provided with each batch?

A: Batch-specific COA, HPLC report, UV/IR identity records, melting point data, MSDS/SDS and relevant export documentation are available according to order requirements.

Q7: What purity do you supply?

A: Standard ≥98% HPLC; typical batch result 99%. Higher grades available on request.

Q8: Where can I buy it from China?

A: HDM BIO supplies bulk Acriflavine Hydrochloride Powder with batch COA and global export support.

Q9: How much does Acriflavine Hydrochloride Powder cost?

A: Bulk pricing depends on synthesis costs, purity grade, regulatory compliance, order volume and logistics. Contact HDM BIO for a current quotation.

Q10: Is acriflavine safe for all uses?

A: Acriflavine is cytotoxic at high concentrations due to DNA binding and may stain surfaces. It is supplied as veterinary raw material only; handle with appropriate PPE and comply with local use regulations. Not for direct clinical administration.