Thymosin Beta‑4 Powder CAS 77591‑33‑4 | High Purity 43‑mer Actin‑Sequestering Regenerative Research Peptide Supplier China

Core Selling Points

✅ ≥98% HPLC Purity Thymopentin Powder with Batch‑Specific COA Available

✅ Verified by HPLC, LC‑MS and Amino Acid Composition Analysis for 5‑mer short‑chain peptide identity confirmation

✅ China Peptide Manufacturer with Fmoc‑SPPS Production & GMP‑Aligned Quality System

✅ Specialized in short‑chain peptide deletion impurity & racemization by‑product control

✅ Complete Documentation Package: COA, HPLC Chromatogram, LC‑MS Report & MSDS

✅ Flexible Supply from Research Samples to Bulk Peptide Orders Worldwide

What Is Thymosin Beta‑4 Powder?

Thymosin Beta‑4 powder, also known as Tβ4, is a synthetic N‑terminally acetylated 43‑amino‑acid endogenous peptide, well‑known for G‑actin sequestering activity, widely studied in cell migration, angiogenesis, anti‑inflammation and tissue‑repair pre‑clinical assays. Sequence: Ac‑Ser‑Asp‑Lys‑Pro‑Asp‑Met‑Ala‑Glu‑Ile‑Glu‑Lys‑Phe‑Asp‑Lys‑Ser‑Lys‑Leu‑Lys‑Lys‑Thr‑Glu‑Thr‑Gln‑Glu‑Lys‑Asn‑Pro‑Leu‑Pro‑Ser‑Lys‑Glu‑Thr‑Ile‑Glu‑Gln‑Glu‑Lys‑Gln‑Ala‑Gly‑Glu‑Ser‑OH, molecular weight 4963.44 Da.

Manufactured via optimized long‑sequence Fmoc solid‑phase peptide synthesis under our GMP‑aligned quality system, our Thymosin Beta‑4 powder strictly controls peptide‑chain aggregation, incomplete coupling deletion fragments, N‑terminal acetylation efficiency and methionine oxidation impurities, securing consistent physicochemical property and batch‑to‑batch reproducibility. HDM BIO serves global biochemistry laboratories, pharmaceutical R&D institutions and biological‑reagent manufacturers, covering milligram‑level research samples up to kilogram‑scale worldwide bulk procurement. This peptide raw material is restricted exclusively to laboratory research use and must not be directly used for human clinical administration.

What Are the Official Quality Specifications of Thymosin Beta‑4 Powder?

Every production batch of Thymosin Beta‑4 undergoes multi‑level QC testing including HPLC, LC‑MS and amino acid composition analysis within HDM BIO in‑house analytical laboratory. Batch‑specific COA will be issued for all purchase orders.

Item	Specification	Result
Appearance	White to off-white powder	Consistent
Purity (HPLC)	≥98%	99.63%
MS Identity	Consistent with reference standard	Confirmed
Conclusion	Cosmetic raw material requirement	Passed

Thymosin Beta‑4 vs Related Thymus‑Family Peptides

For buyers sourcing thymus‑derived bioactive peptide raw materials for regenerative pre‑clinical research, the comparison table clarifies core differences for targeted raw‑material selection：

Product	Target Mechanism	Structural Feature	Primary Research Application	CAS Number
Thymosin Beta‑4	G‑actin sequestering, cell‑migration & angiogenesis modulator	43‑mer long‑chain peptide, N‑terminal acetylation modification	Wound healing, cardiac‑protection, cytoskeleton biology research	77591‑33‑4
Thymopentin	T‑lymphocyte immune regulator	5‑mer short‑chain peptide, no modification	Immunomodulation & lymphocyte function assay	69558‑55‑0

This section covers long‑tail keywords: thymosin beta‑4 Tβ4 raw material, 43‑mer actin‑binding research peptide, N‑acetylated thymus peptide API.

How to Buy Thymosin Beta‑4 Powder from a Trustworthy Supplier?

When sourcing Thymosin Beta‑4 powder for regenerative medicine pre‑clinical research, R&D teams and international purchasers need to assess five core criteria to avoid supply‑quality risks:

HPLC purity validation: Confirm ≥98% HPLC purity with batch‑specific COA and full impurity chromatogram; pay special attention to methionine oxidation, long‑chain deletion fragments and incomplete N‑acetylation impurities which will interfere with cell migration and actin‑binding assay results
Multi‑method structural confirmation: Peptide identity validated by HPLC, LC‑MS and amino‑acid composition analysis to confirm intact full‑length 43‑mer N‑acetylated peptide
Complete batch documentation package: Guarantee each order obtains batch‑specific COA, HPLC chromatogram, LC‑MS report and MSDS to satisfy global import customs clearance and lab audit requirements
Qualified manufacturing system: Select China peptide manufacturer with mature long‑sequence Fmoc‑SPPS workflow and GMP‑aligned quality system, proven track record on long‑chain peptide aggregation‑suppression and N‑terminal modification control technology
Global flexible supply: Confirm vendor can deliver both small‑scale research samples and large‑volume worldwide bulk peptide orders

Thymosin Beta‑4 Powder Price & Bulk Ordering Guide 2026

If you are searching for Thymosin Beta‑4 powder price, bulk quotation and delivery terms, please refer to key procurement information as follows:

Minimum Order Quantity: Flexible MOQ, milligram & gram‑scale research samples available; kilogram‑level worldwide bulk supply for sustained research projects
Sample Access: Trial research samples for lab evaluation; contact sales team to get formal sample quotation
Bulk Kilogram Pricing: Tiered pricing structure; larger bulk volume brings competitive unit price
Production Lead Time: Long‑chain 43‑mer peptide with multi‑step coupling & modification process; sales will confirm exact production schedule upon formal inquiry
Shipping Options: Sealed cold‑recommended shipping worldwide; avoid prolonged room‑temperature exposure; we supply complete documentation package (batch‑specific COA, HPLC chromatogram, LC‑MS report, MSDS, commercial invoice, packing list) for global customs clearance

Thymosin Beta‑4 Powder Procurement Guide for Global Buyers

Before placing purchase orders for long‑chain acetylated regenerative peptide raw materials, international procurement teams should finish these verification steps to mitigate potential risks:

Supplier qualification check: Confirm the manufacturer owns independent peptide‑synthesis workshop and QC lab with GMP‑aligned quality system, hands‑on experience on long‑sequence SPPS synthesis, aggregation‑suppression and N‑terminal acetylation modification
Document inspection: Request pre‑shipment complete documentation package including batch‑specific COA, HPLC chromatogram, LC‑MS report and MSDS; require multi‑method peptide identity confirmation and impurity profile covering methionine oxidation, deletion‑truncated fragments and incomplete acetylation impurities
Product confirmation: Confirm purity standard, endotoxin level and cold‑storage requirement match your experimental design; note long‑chain lyophilized peptide is susceptible to oxidation especially after reconstitution
MOQ & lead‑time confirmation: Verify research sample / bulk peptide worldwide availability, long‑peptide production cycle and order‑adjustment policy
Shipping‑document confirmation: Make sure supplier can provide complete paperwork set to satisfy import requirements of your target region

About HDM BIO Thymosin Beta‑4 Manufacturing Capability

HDM BIO possesses more than 10‑year expertise in Fmoc‑SPPS peptide synthesis under GMP‑aligned quality system, specialized in long‑chain modified bioactive peptide manufacturing with aggregation‑risk control.

Dedicated Fmoc‑SPPS production lines; adopt pseudoproline dipeptide building blocks & optimized coupling protocol to mitigate on‑resin peptide aggregation for long 43‑mer sequence
Strict controlled N‑terminal acetylation reaction to guarantee high modification ratio; monitor methionine oxidation throughout synthesis, cleavage and purification workflow
Independent analytical laboratory equipped with HPLC, LC‑MS and amino‑acid composition analysis instruments; specialized RP‑HPLC gradient workflow for separation of truncated fragments, oxidized variants and non‑acetylated peptide impurities
Peptide products exported worldwide to over 70 countries, supporting university labs, biotech startups and pharmaceutical R&D institutes
Stable annual production capacity for research‑grade peptide raw materials, supporting long‑term framework cooperation
Professional technical team experienced in thymus‑family peptide‑series synthesis and related analog custom modification

What Technical Challenges Exist in Thymosin Beta‑4 Synthesis & Production?

Thymosin Beta‑4 is a 43‑mer long‑chain N‑acetylated peptide, contains easily‑oxidized methionine residue and multiple charged amino‑acids. Controlling on‑resin peptide aggregation, removing deletion fragments, suppressing Met‑oxidation and ensuring full N‑terminal acetylation constitute core technical bottlenecks separating qualified peptide manufacturers from low‑grade suppliersResearchGa....

Key production challenges:

‑ Long 43‑mer sequence tends toward intramolecular aggregation during solid‑phase coupling, leading to incomplete amino‑acid addition and massive deletion‑truncated impurities

‑ Methionine residue is highly susceptible to oxidation during cleavage, purification and lyophilization; oxidized Met variant changes biological activity of actin‑sequestering function

‑ N‑terminal acetylation reaction needs precise stoichiometry and condition‑control; non‑acetylated by‑product shows close HPLC retention time, hard for baseline separation ‑ Multiple acidic & basic charged residues bring complex solubility fluctuation during preparative‑HPLC purification

‑ Single HPLC test is insufficient for quality judgement; multi‑validation via HPLC, LC‑MS plus amino‑acid composition analysis is mandatory to guarantee intact, correctly‑acetylated full‑length 43‑mer peptide for cytoskeleton‑related functional biology assays

How Does Thymosin Beta‑4 Powder Work in Laboratory Research?

As synthetic N‑acetylated long‑chain thymus‑origin regenerative peptide, Thymosin Beta‑4 powder binds and sequesters G‑actin monomers, regulates cytoskeleton remodeling, promotes cell migration, angiogenesis and exerts multi‑target anti‑inflammatory effects. It serves as core experimental reagent for actin‑binding profiling, wound‑healing animal‑model assay, cardiac‑protection pre‑clinical evaluation and thymus‑peptide structure‑activity‑relationship exploration. All mechanism descriptions are limited to in‑vitro and pre‑clinical animal‑research scenarios only.

How Is Thymosin Beta‑4 Powder Manufactured at HDM BIO?

Thymosin Beta‑4 requires strict full‑process control for long‑sequence aggregation suppression, N‑terminal acetylation, methionine‑oxidation prevention and lyophilization, operated within our GMP‑aligned quality system.

Read related guide: Disulfide‑Containing Peptide API Manufacturing Guide | Fmoc SPPS Technology Explained

Complete Thymosin Beta‑4 Powder Manufacturing Workflow

Raw‑material QC of Fmoc‑amino‑acid building blocks (pseudoproline dipeptide & Met side‑chain protection inspection) → Step‑wise 43‑mer peptide SPPS coupling with aggregation‑suppression protocol on solid resin → Resin cleavage & global deprotection under low‑oxidation scavenger system → Controlled N‑terminal acetylation → Crude peptide pretreatment under inert atmosphere → Multi‑stage preparative‑HPLC purification (remove deletion‑fragment impurities, methionine‑oxidation variants & non‑acetylated by‑products) → Buffer‑exchange → Vacuum lyophilization under oxygen‑free environment → Full multi‑method QC testing (HPLC, LC‑MS, amino‑acid composition analysis for identity verification, impurity profiling & endotoxin test) → Batch‑specific COA issuance → Light‑proof, low‑moisture sealed packaging under nitrogen purge

QC Inspection Workflow

Incoming raw‑material QC → In‑process aggregation risk monitoring & acetylation reaction monitoring → HPLC purity testing (long‑chain peptide resolving gradient method) → LC‑MS molecular‑weight & full‑sequence & acetylation‑modification verification → Supplementary amino‑acid composition analysis for peptide identity confirmation → Met‑oxidation & deletion‑impurity & incomplete‑acetylation quantitative analysis → Moisture & heavy‑metal testing → Residual‑solvent & endotoxin detection → Final batch‑release approval under GMP‑aligned quality system

Core Process Highlights

‑ Pseudoproline dipeptide strategy to relieve on‑resin aggregation of long 43‑mer peptide ‑ Oxygen‑free workflow throughout cleavage, purification and lyophilization to suppress methionine oxidation ‑ Optimized N‑terminal acetylation condition to minimize non‑acetylated impurity ‑ Optimized RP‑HPLC gradient for baseline separation of deletion truncated fragments and oxidized variants ‑ Multi‑level identity confirmation: HPLC, LC‑MS plus amino‑acid composition analysis verifying intact N‑acetylated full‑length 43‑mer Thymosin Beta‑4 peptide ‑ Lyophilized solid stable stored at −20 °C sealed under nitrogen; avoid repeated freeze‑thaw cycles, reconstituted peptide solution shall be aliquoted and stored at −80 °C, strictly protect sample from oxygen exposure.

What Are the Main Application Scenarios of Thymosin Beta‑4 Powder?

Cytoskeleton & actin‑biology research: G‑actin sequestering assay, cell migration and cell‑motility mechanism study
Tissue‑repair & wound‑healing pre‑clinical research: Skin injury model, corneal repair, angiogenesis efficacy evaluation
Cardioprotective pre‑clinical research: Myocardial‑ischemia‑reperfusion injury animal‑model research
Anti‑inflammatory biology research: Inflammatory response modulation, oxidative‑stress protection assay
Peptide formulation development: Long‑chain acetylated peptide stability screening, injectable peptide pre‑formulation research
Biomedical reagent preparation: Laboratory reference standards for thymus‑series peptide research
Long‑chain modified‑peptide‑process‑development research: N‑acetylated 43‑mer peptide synthesis benchmark compound

Why Choose HDM BIO As Your Thymosin Beta‑4 Powder Supplier?

‑ 10+‑year China‑based peptide‑manufacturing experience with Fmoc‑SPPS production and GMP‑aligned quality system

‑ Specialized capability for complex long‑chain Thymosin Beta‑4 peptide synthesis and purification, effectively reduce aggregation‑caused deletion fragments, methionine‑oxidation variants and incomplete‑acetylation impurities

‑ Every batch supported by multi‑validation: HPLC, LC‑MS and amino‑acid composition analysis, delivered with batch‑specific complete documentation package

‑ Complete batch‑wise production‑traceability documents for Thymosin Beta‑4 Powder

‑ Flexible worldwide supply capacity: research samples to kilogram‑scale bulk peptide orders

‑ Global‑export service covering more than 70 countries worldwide

‑ Professional technical team supporting thymus‑family peptide research and custom‑analog synthesis consulting

FAQs About Thymosin Beta‑4 Powder

Q: Where can I buy Thymosin Beta‑4 powder from China?

A: HDM BIO is a Chinese peptide manufacturer with Fmoc‑SPPS production and GMP‑aligned quality system, specialized in long‑chain N‑acetylated thymus‑derived research peptide. We deliver high‑purity Thymosin Beta‑4 powder for global pharmaceutical R&D teams and bioreagent laboratories, flexible worldwide supply from research samples to bulk peptide orders.

Q: What documents should a qualified Thymosin Beta‑4 supplier provide?

A: Reliable supplier shall supply complete documentation package: batch‑specific COA, HPLC chromatogram, LC‑MS report & MSDS. Peptide identity is verified by HPLC, LC‑MS and amino‑acid composition analysis; methionine oxidation and incomplete‑acetylation impurity‑testing data are available upon request.

Q: What is the CAS number of Thymosin Beta‑4 Powder?

A: The CAS registry number of Thymosin Beta‑4 Powder is 77591‑33‑4.

Q: What purity grade of Thymosin Beta‑4 powder can you deliver?

A: Standard research‑grade ≥98% HPLC purity. Every batch comes with batch‑specific COA and multi‑method identity validation including amino‑acid composition check. Higher‑purity specifications and thymus‑series custom‑peptide‑analog synthesis are available for special experimental requirements.

Q: How should lyophilized Thymosin Beta‑4 powder be stored?

A: Store sealed under nitrogen atmosphere at −20 °C, keep away from light, moisture and oxygen. Strictly avoid repeated freeze‑thaw cycles. Aqueous reconstituted solution is susceptible to methionine oxidation; prepare single‑use aliquots and store at −80 °C, long‑term storage under 2‑8 °C is not recommended.

Q: Can I get Thymosin Beta‑4 research samples before bulk purchase?

A: Yes. We provide milligram‑to‑gram‑level research samples attached with complete documentation package for laboratory assessment.

Q: What is the MOQ for bulk Thymosin Beta‑4 powder worldwide orders?

A: MOQ is flexible depending on purity grade and packaging specifications. We support small‑scale research samples and large‑volume worldwide bulk peptide orders for different‑project requirements.

Q: What is the amino‑acid sequence of Thymosin Beta‑4 Powder?

A:Ac‑Ser‑Asp‑Lys‑Pro‑Asp‑Met‑Ala‑Glu‑Ile‑Glu‑Lys‑Phe‑Asp‑Lys‑Ser‑Lys‑Leu‑Lys‑Lys‑Thr‑Glu‑Thr‑Gln‑Glu‑Lys‑Asn‑Pro‑Leu‑Pro‑Ser‑Lys‑Glu‑Thr‑Ile‑Glu‑Gln‑Glu‑Lys‑Gln‑Ala‑Gly‑Glu‑Ser‑OH (N‑terminal acetylation modification).

Scientific Review

Reviewed by:

HDM BIO Peptide Technical Team

Expertise:

Fmoc‑SPPS peptide synthesis Disulfide‑bond‑containing cyclic hormone peptide manufacturing Multi‑method peptide characterization: HPLC, LC‑MS and disulfide‑bond verification

Last Updated: August 2026

Disclaimer: All product content serves academic research and laboratory raw‑material reference only. Not for human clinical use, diagnosis or treatment.